Cell Plasma Membrane Staining Kit (Green Fluorescence)
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The fluorescence images of HL-60 cells by Cellpaint Green co-stained with Hoechst 33342 on a glass slide.
The fluorescence images of HL-60 cells by Cellpaint Green co-stained with Hoechst 33342 on a glass slide.
The cells were imaged using fluorescence microscope using 60X oil lens with a FITC filter. The images appeared to be more punctured and diffused since all the fluorescence signals from membranes both above and below the cell borders were indiscriminately captured. Compared to regular epifluorescence imaging, confocal fluorescence imaging is more sensitive and may give you more control than a regular epifluorescence microscope. If you have access to a confocal microscope, you may get a crisper imaging of cell membranes.
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Specification
Product Description
Cell Plasma Membrane Staining Kit (Green Fluorescence) provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins. It may be used as a segmentation tool for HCS (high-content screening), as well as to stain cellular plasma membranes for standard fluorescence microscopy. The green fluorescent stain used in the kit survives fixation, but not permeabilization, so it is not suitable for experiments that also involve probing internal targets via antibodies.
Suitable Sample
Cells.
Instrument
Fluorescence microscope
Excitation
FITC filter set
Emission
FITC filter set
Recommended plate
Black wall/clear bottom/Glass slides
Regulatory Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C and avoid from light.
Note
The fluorescence images of HL-60 cells by Cellpaint Green co-stained with Hoechst 33342 on a glass slide.
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Applications
Functional Study
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