Factor Xa (Human) Chromogenic Activity Assay Kit
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The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
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Specification
Product Description
Factor Xa (Human) Chromogenic Activity Assay Kit is developed to determine FXa activity in human plasma, serum, and cell culture samples. The assay couples immunofunctional and direct amidolytic function.
Suitable Sample
Cell Culture Supernatants, Plasma and Serum.
Sample Volume
100 uL
Calibration Range
0.031 to 2.0 mIU/mL or 0.141 to 9.0 ng/mL
Reactivity
Human
Regulatory Status
For research use only (RUO)
Quality Control Testing
Standard curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store components of the kit at 4°C or -20°C as described in the protocol.
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Applications
Functional Study
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Gene Info — F10
Entrez GeneID
2159Protein Accession#
P00742Gene Name
F10
Gene Alias
FX, FXA
Gene Description
coagulation factor X
Omim ID
227600Gene Ontology
HyperlinkGene Summary
This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq
Other Designations
OTTHUMP00000018735|Stuart-Prower factor|factor Xa|prothrombinase
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