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B4GALT1 (Human) ELISA Kit 

  • Catalog # : KA5762
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  • Specification
  • Product Description:
  • B4GALT1 (Human) ELISA Kit is a sandwich enzyme-linked immunosorbent assay for the quantitative measurement of human B4GALT1.
  • Calibration Range:
  • 0.78 to 50 ng/mL
  • Reactivity:
  • Human
  • Quality Control Testing:
  • Standard curve

    QC Testing of KA5762
    The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
  • Storage Instruction:
  • Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
  • Suitable Sample:
  • Cell Culture Supernates, Plasma (EDTA, Heparin), and Serum.
  • Sample Volume:
  • 100 uL
  • Label:
  • HRP-conjugated
  • Detection Method:
  • Colorimetric
  • Regulatory Status:
  • For research use only (RUO)
  • Applications
  • Quantification
  • Application Image
  • Quantification
  • Gene Information
  • Entrez GeneID:
  • 2683
  • Gene Name:
  • B4GALT1
  • Gene Alias:
  • B4GAL-T1,DKFZp686N19253,GGTB2,GT1,GTB,MGC50983,beta4Gal-T1
  • Gene Description:
  • UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
  • Gene Summary:
  • This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000021196,UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 1, membrane-bound form,glycoprotein-4-beta-galactosyltransferase 2,lactose synthase A protein
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