Cell-based Cholesterol Efflux Assay Kit (Fluorometric) 

Percentage (%) Labeled Cholesterol Efflux. J774.1 cells were labeled with the fluorescent cholesterol Labeling Media and treated with various cholesterol acceptors such as 2% LDL/VLDL-depleted Human Serum (2 uL/well), purified human HDL (25 ug/mL, 2.5 ug/well) or Positive Control reagent. Cholesterol efflux is expressed as percentage of fluorescently-labeled cholesterol transferred from labeled adherent macrophages to designated cholesterol acceptor present in supernatant over a 4 hour time period, with no treatment control (efflux to medium containing no acceptor) percentage subtracted (mean plus minus SD of N = 5-6 wells).