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Last updated: 2022/5/22

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Cell-based Cholesterol Efflux Assay Kit (Fluorometric) 

  • Catalog # : KA4542
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  • Specification
  • Product Description:
  • Cell-based Cholesterol Efflux Assay Kit (Fluorometric) is a high-throughput screening assay for measuring cholesterol efflux from cells using a fluorescently-labeled cholesterol analogue.
  • Reactivity:
  • Mammals
  • Storage Instruction:
  • Store the kit at -20°C, protected from light.
  • Note:
  • Result Data
  • Percentage (%) Labeled Cholesterol Efflux. J774.1 cells were labeled with the fluorescent cholesterol Labeling Media and treated with various cholesterol acceptors such as 2% LDL/VLDL-depleted Human Serum (2 uL/well), purified human HDL (25 ug/mL, 2.5 ug/well) or Positive Control reagent. Cholesterol efflux is expressed as percentage of fluorescently-labeled cholesterol transferred from labeled adherent macrophages to designated cholesterol acceptor present in supernatant over a 4 hour time period, with no treatment control (efflux to medium containing no acceptor) percentage subtracted (mean plus minus SD of N = 5-6 wells).

  • Suitable Sample:
  • Human Serum, Purified HDL Particles, Isolated or Recombinant Lipoproteins.
  • Detection Method:
  • Fluorometric
  • Regulation Status:
  • For research use only (RUO)
  • Publication Reference
  • Applications
  • Functional Study
  • Application Image
  • Functional Study
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