SIRT3 Deacetylase Fluorometric (Human) Assay Kit is designed for the rapid and sensitive evaluation of SIRT3 inhibitors or activators using crude SIRT3 fraction or purified SIRT3. Additionally, any cultured primary cell, cell line, or tissue homogenate can be assayed for SIRT3 activity with the SIRT3 deacetylase fluorometric assay kit after immunoprecipitation with an appropriate SIRT3 specific antibody.
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant SIRT3 activity.
Store the kit at -80°C. Do not expose reagents to excessive light.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq
mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase,silent mating type information regulation 2, S.cerevisiae, homolog 3,sir2-like 3,sirtuin 3,sirtuin type 3