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Detection of phosphatidylserine binding activity in Jurkat cells. Jurkat cells were treated without (Blue) or with 20 uM camptothecin (red) in a 37°C, 5% CO2 incubator for 4-5 hours, and then loaded with Apopxin Deep Red for 30 minutes. The fluorescence intensity of Apopxin Deep Red was measured with a FACSCalibur flow cytometer in FL4 channel.