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Detection of Apopxin Violet 450-PS binding activity in Jurkat cells. Jurkat cells were seeded on the same day at 200,000 cells/90 L/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1 uM staurosporine for 5 hours. The Apopxin Violet 450 assay solution (100 uL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 405/450 nm with FlexStation (from Molecular Devices) using bottom read mode.