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The detection of binding activity of Apopxin Violet 450 and phosphatidylserine in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 uM staurosporine (Red) in a 37°C, 5% CO2 incubator for 5 hours, and then dye loaded with Apopxin Violet 450 for 30 minutes. The fluorescence intensity of Apopxin Violet 450 was measured with a FACSCalibur flow cytometer using violet laser at Ex/Em = 405/450 nm.