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The increase in fluorescence intensity of Nuclear Green DCS1 with the addition of Camptothecin in Jurkat cells. Jurkat cells were treated overnight without (A) or with 20 uM camptothecin (B) in a 37°C, 5% CO2 incubator, and then dye loaded with Nuclear Green DCS1 for 60 minutes. At the end of 15 minutes of Nuclear Green DCS1 dye loading, MitoLite NIR was added for multicolor analysis. The fluorescence intensity of Nuclear Green DCS1 and MitoLite NIR was measured with a FACSCalibur flow cytometer using FL1 channel (Nuclear Green DCS1) and FL4 channel (MitoLite NIR).