NIR Mitochondrial Membrane Potential Assay Kit
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Result Data
Result Data
The decrease in MitoLite NIR fluorescence with the addition of FCCP in Hela cells. Hela cells were dye loaded with MitoLite NIR alone or in the presence of 20 uM FCCP for 15 minutes. The fluorescence intensity of MitoLite NIR was measured 30 minutes after adding Assay buffer B (Component C) with a FlexStation microplate reader (Molecular Devices) at Ex/Em = 640/680 nm (cut off 665 nm, bottom read).
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Specification
Product Description
NIR Mitochondrial Membrane Potential Assay Kit is a fluorescent assay used to detect cell apoptosis by monitoring the loss of mitochondiral membrane potential with microplate readers.
Suitable Sample
Adherent cells, Non-adherent cells
Detection Method
Fluorometric
Excitation (Max)
640 nm
Emission (Max)
680 nm
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C and avoid from light.
Note
Result Data
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Applications
Functional Study
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