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The decrease in MitoLite NIR fluorescence with the addition of FCCP in Hela cells. Hela cells were dye loaded with MitoLite NIR alone or in the presence of 20 uM FCCP for 15 minutes. The fluorescence intensity of MitoLite NIR was measured 30 minutes after adding Assay buffer B (Component C) with a FlexStation microplate reader (Molecular Devices) at Ex/Em = 640/680 nm (cut off 665 nm, bottom read).