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Last updated: 2017/6/25

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Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence)

  • Catalog # : KA4156
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  • Specification
  • Product Description:
  • Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence) is a fluorescent assay used to detect cell apoptosis by monitoring the loss of mitochondiral membrane potential with microplate readers.
  • Storage Instruction:
  • Store the kit at -20°C and avoid from light.
  • Note:
  • Result Data
  • The decrease in MitoLite Orange fluorescence with the addition of FCCP in Hela cells. Hela cells were dye loaded with MitoLite Orange alone or in the presence of 20 uM FCCP for 15 minutes. The fluorescence intensity of MitoLite Orange was measured 30 minutes after adding Assay Buffer B (Component C) with a FlexStation microplate reader (Molecular Devices) at Ex/Em = 540/590 nm (cut off 570 nm, bottom read).


  • Suitable Sample:
  • Adherent cells, Non-adherent cells
  • Detection Method:
  • Fluorometric
  • Excitation (Max):
  • 540 nm
  • Emission (Max):
  • 590 nm
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Functional Study
  • Application Image
  • Functional Study
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