Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence)
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Result Data
Result Data
The decrease in fluorescence intensity of MitoLite Orange with the addition of FCCP in Jurkat cells. Jurkat cells were loaded with MitoLite Orange alone (Blue) or in the presence of 30 uM FCCP (Red) for 15 minutes. The fluorescence intensity of MitoLite Orange was measured with a FACSCalibur flow cytometer using FL2 channel.
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Specification
Product Description
Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence) is a fluorescent assay used to detect cell apoptosis by monitoring the loss of mitochondiral membrane potential with flow cytometry.
Suitable Sample
Cells
Detection Method
Fluorometric
Excitation (Max)
540 nm
Emission (Max)
590 nm
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C and avoid from light.
Note
Result Data
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Applications
Functional Study
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Publication Reference
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ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells.
Tan BL, Norhaizan ME, Chan LC.
Evidence-Based Complementary and Alternative Medicine 2018 Jun; 2018:6578648.
Application：Func, Human, HeLa cells.
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ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells.
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