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Campotothecin-induced mitochondria membrane potential changes were measured with JC-10 and JC-1 in Jurkat cells. After Jurkat cells were treated with camptothecin (10 mM) for 4 hours, JC-1 and JC-10 dye loading solutions were added to the wells and incubated for 30 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-1 and JC-10 were measured at Ex/Em = 490/525 nm and 490/590 nm with NOVOstar microplate reader.