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Effect of FCCP induced mitochondria membrane potential change in JurKat cells. JurKat cells were dye loaded with JC-10 dye-loading solution along with DMSO (Top) or 5 uM FCCP (Low) for 10 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-10 were measured with a FACSCalibur flow cytometer using FL1 and FL2 channels. Uncompensated data (left column) were compared with compensated data (right column).