JC-10 Mitochondrial Membrane Potential Assay Kit
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Result Data
Result Data
Effect of FCCP induced mitochondria membrane potential change in JurKat cells. JurKat cells were dye loaded with JC-10 dye-loading solution along with DMSO (Top) or 5 uM FCCP (Low) for 10 minutes. The fluorescent intensities for both J-aggregates and monomeric forms of JC-10 were measured with a FACSCalibur flow cytometer using FL1 and FL2 channels. Uncompensated data (left column) were compared with compensated data (right column).
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Specification
Product Description
JC-10 Mitochondrial Membrane Potential Assay Kit is a fluorescent assay used to detect cell apoptosis by monitoring the loss of mitochondiral membrane potential with flow cytometry.
Detection Method
Fluorometric
Excitation/<br>Emission (nm)
510/525
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C and avoid from light.
Note
Result Data
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Applications
Functional Study
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Publication Reference
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Lauric acid alleviates insulin resistance by improving mitochondrial biogenesis in THP-1 macrophages.
Yong Yao Tham, Quok Cheong Choo, Tengku Sifzizul Tengku Muhammad, Choy Hoong Chew.
Molecular Biology Reports 2020 Dec; 47(12):9595.
Application：Flow Cyt, Human, THP-1 cells.
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Lauric acid alleviates insulin resistance by improving mitochondrial biogenesis in THP-1 macrophages.
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