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CHO-K1 cell number response was measured with Cell Cytotoxicity Assay Kit (Fluorometric). CHO-K1 cells at 0 to 10,000 cells/well/100 uL were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 20 uL/well of Assay Solution (Component A) for 3 hours at 37°C. The fluorescence intensity was measured at Ex/Em = 540/590 nm with NOVOstar instrument (BMG Labtech). The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated. The detection limit was 60 cells/well (n=6). The insert shows the enlargement of the lower end of the cell number response.