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Last updated: 2017/12/10

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Cell Cytotoxicity Assay Kit (Fluorometric)

  • Catalog # : KA4152
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  • Specification
  • Product Description:
  • Cell Cytotoxicity Assay Kit (Fluorometric) is a non-radioactive and no-wash method assay suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.
  • Limit of Detection:
  • 100 cells
  • Storage Instruction:
  • Store the kit at -20°C and avoid from light.
  • Note:
  • Result Data
  • CHO-K1 cell number response was measured with Cell Cytotoxicity Assay Kit (Fluorometric). CHO-K1 cells at 0 to 10,000 cells/well/100 uL were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 20 uL/well of Assay Solution (Component A) for 3 hours at 37°C. The fluorescence intensity was measured at Ex/Em = 540/590 nm with NOVOstar instrument (BMG Labtech). The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated. The detection limit was 60 cells/well (n=6). The insert shows the enlargement of the lower end of the cell number response.

  • Suitable Sample:
  • Cells
  • Excitation (Max):
  • 540 nm
  • Applications
  • Functional Study
  • Application Image
  • Functional Study
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