Cell Cytotoxicity Assay Kit (Fluorometric)
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Result Data
Result Data
CHO-K1 cell number response was measured with Cell Cytotoxicity Assay Kit (Fluorometric). CHO-K1 cells at 0 to 10,000 cells/well/100 uL were seeded overnight in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 20 uL/well of Assay Solution (Component A) for 3 hours at 37°C. The fluorescence intensity was measured at Ex/Em = 540/590 nm with NOVOstar instrument (BMG Labtech). The fluorescence intensity was linear (R2 = 0.998) to the cell number as indicated. The detection limit was 60 cells/well (n=6). The insert shows the enlargement of the lower end of the cell number response.
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Specification
Product Description
Cell Cytotoxicity Assay Kit (Fluorometric) is a non-radioactive and no-wash method assay suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.
Suitable Sample
Cells
Excitation (Max)
540 nm
Limit of Detection
100 cells
Storage Instruction
Store the kit at -20°C and avoid from light.
Note
Result Data
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Applications
Functional Study
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