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The Effect of Jurkat cells on Saponin induced cell death measured with Cell Viability Assay Kit (Green/Red Dual Fluorescence). Jurkat cells at 2 X106 cells/mL were treated with or without 0.5% Saponin for 5 minutes, then centrifuged and the supernatant were replaced with fresh medium. 100 uL of untreated cells (A), 50 uL each of untreated and treated cells (B), 25 uL of untreated and 75 uL treated cells (C), and 100 uL of 0.5% saponin treated cells (D) were plated in a 96-well black wall/clear bottom Poly-D-lysine plate. The cells were incubated with 100 uL/well of CytoCalcein Green/ Propidium Iodide dye-loading solution for 1 hr at 37°C. The fluorescence intensity was measured at Ex/Em = 490/525 nm and 540/650 nm using NOVOstar instrument (BMG Labtech). The ratio of 490/525 nm to 540/650 nm fluorescence intensity on live and dead cells were showed as indicated (n=6).