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Last updated: 2021/4/11

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Cell Viability Assay Kit (Green/Red Dual Fluorescence) 

  • Catalog # : KA4149
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  • Specification
  • Product Description:
  • Cell Viability Assay Kit (Green/Red Dual Fluorescence) is a dual fluorescent assay used to monitor cell viability and cellular functions. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
  • Storage Instruction:
  • Store the kit at -20°C and avoid from light.
  • Note:
  • Result Data
  • The Effect of Jurkat cells on Saponin induced cell death measured with Cell Viability Assay Kit (Green/Red Dual Fluorescence). Jurkat cells at 2 X106 cells/mL were treated with or without 0.5% Saponin for 5 minutes, then centrifuged and the supernatant were replaced with fresh medium. 100 uL of untreated cells (A), 50 uL each of untreated and treated cells (B), 25 uL of untreated and 75 uL treated cells (C), and 100 uL of 0.5% saponin treated cells (D) were plated in a 96-well black wall/clear bottom Poly-D-lysine plate. The cells were incubated with 100 uL/well of CytoCalcein Green/ Propidium Iodide dye-loading solution for 1 hr at 37°C. The fluorescence intensity was measured at Ex/Em = 490/525 nm and 540/650 nm using NOVOstar instrument (BMG Labtech). The ratio of 490/525 nm to 540/650 nm fluorescence intensity on live and dead cells were showed as indicated (n=6).

  • Suitable Sample:
  • Cells
  • Detection Method:
  • Fluorometric
  • Platform:
  • Instrument: Fluorescence microplate reader
    Excitation: 490 nm (live), 540 nm (dead)
    Emission: 525 nm (live), 620 nm (dead)
    Cutoff: 515 nm, 590 nm
    Recommended plate: Solid black

    Instrument: Fluorescence microscope
    Excitation: FITC filter (live), TRITC filter (dead)
    Emission: FITC filter (live), TRITC filter (dead)
    Recommended plate: Black wall/clear bottom

    Instrument: Flow cytometer
    Excitation: 488 nm laser
    Emission: 530/30 nm, 610/20 nm filter
    Instrument specification(s): FITC, PE-Texas Red channel
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Functional Study
  • Application Image
  • Functional Study
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