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Detection of Caspase 9 Activities in Jurkat cells. Jurkat cells were seeded at 200,000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with 1uM staurosporine for 5 hours while the untreated cells were used as control. The caspase 9 assay loading solution (100 uL/well) was added and incubated at room temperature for 1hour. The fluorescence intensity was measured at Ex/Em = 370/450 nm with a FlexStation microplate reader (Molecular Devices).