|Click this icon to add products to compare list. Select up to 10 products.|
Detection of Caspase 3/7 Activities in Jurkat cells. Jurkat cells were seeded on the same day at 80,000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with camptothecin at the final concentration of 20 uM for 5 hours while the untreated cells were used as control. The treated cells and controls were incubated with or without the caspase 3/7 inhibitor AC-DEVD-CHO (5 uM) for 10 minutes. The caspase 3/7 assay solution (100 uL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 490/525 nm with OVOStar instrument (from BMG Labtech).