CASP3/CASP7 Apoptosis Kit (Blue Fluorescence)
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Result Data
Result Data
Detection of Caspase 3/7 Activities in Jurkat cells. Jurkat cells were seeded on the same day at 80,000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with 20 uM camptothecin for 5 hours while the untreated cells were used as control. The treated cells and controls were incubated with or without the caspase 3/7 inhibitor AC-DEVD-CHO (5 uM) for 10 minutes. The caspase 3/7 assay solution (100 uL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 350/450 nm with NOVOStar instrument (from BMG Labtech).
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Specification
Product Description
CASP3/CASP7 Apoptosis Kit (Blue Fluorescence) is a fluorescent assay used to monitor cell apoptosis.
Suitable Sample
Adherent cells, Non-adherent cells
Detection Method
Fluorometric
Excitation (Max)
350 nm
Emission (Max)
450 nm
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C and avoid from light.
Note
Result Data
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Applications
Functional Study
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