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Detection of Caspase 3/7 Activities in Jurkat cells. Jurkat cells were seeded on the same day at 80,000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with 20 uM camptothecin for 5 hours while the untreated cells were used as control. The treated cells and controls were incubated with or without the caspase 3/7 inhibitor AC-DEVD-CHO (5 uM) for 10 minutes. The caspase 3/7 assay solution (100 uL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 350/450 nm with NOVOStar instrument (from BMG Labtech).