CASP3/CASP7 Assay Kit (Red Fluorescence)
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Result Data
Result Data
Detection of Caspase 3/7 Activities in Jurkat cells. Jurkat cells were seeded on the same day at 200,000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with staurosporine at the final concentration of 1M for 5 hours while the untreated cells were used as control. The caspase 3/7 assay solution (100 uL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 540/620 nm with FlexStation fluorescence microplate reader (Molecular Devices).
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Specification
Product Description
CASP3/CASP7 Assay Kit (Red Fluorescence) is a fluorescent assay used to detect the activity of Caspase 3 and Caspase 7.
Suitable Sample
Adherent cells, Non-adherent cells
Detection Method
Fluorometric
Excitation (Max)
535 nm
Emission (Max)
620 nm
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C and avoid from light.
Note
Result Data
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Applications
Functional Study
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