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Detection of Caspase 3/7 Activity in Jurkat cells. Jurkat cells were seeded on the same day at 80,000 cells/well/90 uL in a black wall/clear bottom 96-well Costar plate. The cells were treated with or without 20 uM camptothecin for 5 hours. The caspase 3/7 assay solution (100 uL/well) was added and incubated at room temperature for 1 hour. The luorescence intensity was measured at Ex/Em = 350/450 nm using NOVOStar instrument (from BMG Labtech).