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Last updated: 2016/10/23

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Fatty Acid Uptake Assay Kit

  • Catalog # : KA4084
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  • Specification
  • Product Description:
  • Fatty Acid Uptake Assay Kit is used for the measurement of fatty acid uptake.
  • Storage Instruction:
  • Store the kit in desiccated environment at -20°C and avoid from light.
  • Note:
  • Comparison of fatty acid uptake by 3T3-L1 adipocytes and fibroblast.
  • Cells were plated at 50,000 cells/100uL/well in a 96 well black wall/clear bottom poly-D lysine plate for 5 hours, and then serum deprived for 1 hour. Cells were treated without (control) or with insulin (150 nM), and incubated at 37°C, 5% CO2 incubator for 30 min. At the end of the incubation time, 100 ul of fatty acid mixture was added into the well, and incubated for another 60 min, the fluorescence signal was measured with a FlexStation plate reader using bottom read mode. A: fibroblasts (Control); B: fibroblasts (Insulin); C: adipocytes (Control); D: adipocytes (Insulin). The fluorescence in blank wells with the growth medium is subtracted from the values for the wells with cells. The background fluorescence of the blank wells may vary depending on the sources of the growth media or the microtiter plates.

  • Suitable Sample:
  • Live Cells
  • Excitation/
    Emission (nm):
  • 485/515 nm
  • Detection Method:
  • Fluorometric
  • Instrument Platform:
  • Fluorescence Microscope, Microplate Readers
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Quantification
  • Application Image
  • Quantification
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