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Cells were plated at 50,000 cells/100uL/well in a 96 well black wall/clear bottom poly-D lysine plate for 5 hours, and then serum deprived for 1 hour. Cells were treated without (control) or with insulin (150 nM), and incubated at 37°C, 5% CO2 incubator for 30 min. At the end of the incubation time, 100 ul of fatty acid mixture was added into the well, and incubated for another 60 min, the fluorescence signal was measured with a FlexStation plate reader using bottom read mode. A: fibroblasts (Control); B: fibroblasts (Insulin); C: adipocytes (Control); D: adipocytes (Insulin). The fluorescence in blank wells with the growth medium is subtracted from the values for the wells with cells. The background fluorescence of the blank wells may vary depending on the sources of the growth media or the microtiter plates.