Calcium Assay Kit (Red Fluorescence) is used for the measurement of calcium level in physiology solutions.
30 uM-10 mM
Limit of Detection:
Quality Control Testing:
A calcium standard curve is shown with 96-well black plate and the Calcium Assay Kit (Red Fluorescence) (Cat # KA4080). As low as 0.01 mM calcium can be detected with 5 minutes incubation time (n=3). The fluorescence in blank wells (with H2O only) is used as a control, and is subtracted from the values for those wells with calcium reactions. Note: The fluorescence background increases with time, thus it is important to subtract the fluorescence
intensity value of the blank wells for each data point.
Store the kit in desiccated environment at -20°C and avoid from light.