Membrane Potential Assay Kit (Red Fluorescence)
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ATP dose response in HEK cells transiently transfected with P2X receptor.
ATP dose response in HEK cells transiently transfected with P2X receptor.
HEK cells transiently transfected with P2X receptor were seeded overnight at 40,000 cells/100uL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 uL of the MP dye-loading solution in a 5% CO2, 37°C incubator for 60 minutes. ATP (50 uL/well) was added by FlexStation to achieve the final indicated concentrations. The fluorescence signal was measured with bottom read mode at Ex/Em = 620/650 nm (cutoff at 630 nm).
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Specification
Product Description
Membrane Potential Assay Kit (Red Fluorescence) is used for the measurement of membrane potential.
Suitable Sample
Live Cells
Excitation/<br>Emission (nm)
620/650 nm
Detection Method
Fluorometric
Instrument Platform
FDSS, FlexStation, NOVOStar
Regulation Status
For research use only (RUO)
Storage Instruction
Store components of the kit at 4°C or -20°C and avoid from light as described in the protocol.
Note
ATP dose response in HEK cells transiently transfected with P2X receptor.
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Applications
Quantification
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