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Last updated: 2016/12/4

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Membrane Potential Assay Kit (Red Fluorescence)

  • Catalog # : KA4078
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  • Specification
  • Product Description:
  • Membrane Potential Assay Kit (Red Fluorescence) is used for the measurement of membrane potential.
  • Storage Instruction:
  • Store the kit in desiccated environment at -20°C and avoid from light.
  • Note:
  • ATP dose response in HEK cells transiently transfected with P2X receptor.
  • HEK cells transiently transfected with P2X receptor were seeded overnight at 40,000 cells/100uL/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 uL of the MP dye-loading solution in a 5% CO2, 37°C incubator for 60 minutes. ATP (50 uL/well) was added by FlexStation to achieve the final indicated concentrations. The fluorescence signal was measured with bottom read mode at Ex/Em = 620/650 nm (cutoff at 630 nm).


  • Suitable Sample:
  • Live Cells
  • Excitation/
    Emission (nm):
  • 620/650 nm
  • Detection Method:
  • Fluorometric
  • Instrument Platform:
  • FDSS, FlexStation, FLIPR, NOVOStar
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Quantification
  • Application Image
  • Quantification
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