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Last updated: 2017/12/10

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Autophagy Assay Kit

  • Catalog # : KA4076
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  • Specification
  • Product Description:
  • Autophagy Assay Kit is used for the measurement of autophagy.
  • Storage Instruction:
  • Store the kit in desiccated environment at -20°C and avoid from light.
  • Note:
  • Component A-labeled vesicles are induced by starvation in HeLa cells.
  • Hela cells were incubated in a regular DMEM medium as control (A) or in a serum-depleted medium as autophagy treatment (B) for 16 hours. Both control cells and starved cells were incubated with component A for 30 minutes in a 37°C, 5% CO2 incubator, and then washed four times with wash buffer. Cells were imaged immediately under a fluorescence microscope with a DAPI channel. Autophagy is indicated by bright blue dot staining of autophagic vacuoles (B).

  • Suitable Sample:
  • Live Cells
  • Excitation/
    Emission (nm):
  • 333/518 nm
  • Detection Method:
  • Fluorometric
  • Instrument Platform:
  • Flow Cytometer, Fluorescence Microplate Reader, Fluorescence Microscope
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Quantification
  • Application Image
  • Quantification
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