Autophagy Assay Kit
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Component A-labeled vesicles are induced by starvation in HeLa cells.
Component A-labeled vesicles are induced by starvation in HeLa cells.
Hela cells were incubated in a regular DMEM medium as control (A) or in a serum-depleted medium as autophagy treatment (B) for 16 hours. Both control cells and starved cells were incubated with component A for 30 minutes in a 37°C, 5% CO2 incubator, and then washed four times with wash buffer. Cells were imaged immediately under a fluorescence microscope with a DAPI channel. Autophagy is indicated by bright blue dot staining of autophagic vacuoles (B).
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Specification
Product Description
Autophagy Assay Kit is used for the measurement of autophagy.
Suitable Sample
Live Cells
Excitation/<br>Emission (nm)
330/520 nm
Detection Method
Fluorometric
Instrument Platform
Fluorescence microscope, Fluorescence microplate reader
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit in desiccated environment at -20°C and avoid from light.
Note
Component A-labeled vesicles are induced by starvation in HeLa cells.
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Applications
Quantification
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