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Last updated: 2017/1/15

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Intracellular Total ROS Activity Assay Kit (Orange Fluorescence)

  • Catalog # : KA4075
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  • Specification
  • Product Description:
  • Intracellular Total ROS Activity Assay Kit (Orange Fluorescence) is used for the measurement of intracellular total ROS activity.
  • Storage Instruction:
  • Store the kit in desiccated environment at -20°C and avoid from light.
  • Note:
  • Detection of ROS in Hela cells.
  • Top: Hela cells were seeded overnight at 15,000 cells/90uL/well in a Costar black wall/clear bottom 96-well plate. The cells were untreated (control) or treated with1 mM H2O2 or 100 uM tert-butyl hydroperoxide (TBHP) for 30 min at 37°C. The ROS 570 assay solution (100uL/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. The fluorescence signal were monitored at Ex/Em = 540/570 nm (cut off = 550 nm) with bottom read mode using FlexStation (Molecular Devices).
    Bottom: Images of Hela cells stained with the Intracellular Total ROS Activity Assay Kit (Orange Fluorescence) (Cat # KA4075) in a Costar black wall/clear bottom 96-well plate. A: Untreated control cells. B: Cells treated with 100 uM tert-butyl hydroperoxide (TBHP) for 30min before staining.


  • Suitable Sample:
  • Live Cells
  • Excitation/
    Emission (nm):
  • 540/570 nm
  • Detection Method:
  • Fluorometric
  • Instrument Platform:
  • Flow Cytometer, Fluorescence Microplate Reader, Fluorescence Microscope
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Quantification
  • Application Image
  • Quantification
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