Intracellular Total ROS Activity Assay Kit (Green Fluorescence)
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More Files
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Detection of ROS in Jurkat cells.
Detection of ROS in Jurkat cells.
Jurkat cells were seeded on the same day at 300,000 cells/100uL/well in a Costar black wall/clear bottom 96-well plate. The ROS assay loading solution (100 uL/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. And then the cells were treated with 1mM, 0.1mM H2O2 or without H2O2 for 30 minutes. The fluorescence signal was monitored at Ex/Em = 490/525 nm (cutoff at 515 nm) with bottom read mode using FlexStation (Molecular Devices).
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Specification
Product Description
Intracellular Total ROS Activity Assay Kit (Green Fluorescence) is used for the measurement of intracellular total ROS activity.
Suitable Sample
Live Cells
Excitation/<br>Emission (nm)
490/525 nm
Detection Method
Fluorometric
Instrument Platform
Fluorescence Microplate Reader
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit in desiccated environment at -20°C and avoid from light.
Note
Detection of ROS in Jurkat cells.
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Applications
Quantification
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