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Last updated: 2017/4/23

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Intracellular Total ROS Activity Assay Kit (Green Fluorescence)

  • Catalog # : KA4074
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  • Specification
  • Product Description:
  • Intracellular Total ROS Activity Assay Kit (Green Fluorescence) is used for the measurement of intracellular total ROS activity.
  • Storage Instruction:
  • Store the kit in desiccated environment at -20°C and avoid from light.
  • Note:
  • Detection of ROS in Jurkat cells.
  • Jurkat cells were seeded on the same day at 300,000 cells/100uL/well in a Costar black wall/clear bottom 96-well plate. The ROS assay loading solution (100 uL/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. And then the cells were treated with 1mM, 0.1mM H2O2 or without H2O2 for 30 minutes. The fluorescence signal was monitored at Ex/Em = 490/525 nm (cutoff at 515 nm) with bottom read mode using FlexStation (Molecular Devices).


  • Suitable Sample:
  • Live Cells
  • Excitation/
    Emission (nm):
  • 490/525 nm
  • Detection Method:
  • Fluorometric
  • Instrument Platform:
  • Fluorescence Microplate Reader
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Quantification
  • Application Image
  • Quantification
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