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Last updated: 2016/9/25

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ROS Activity Assay Kit

  • Catalog # : KA3842
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  • Specification
  • Product Description:
  • ROS Activity Assay Kit is a quantitative fluorometric determination of intracellularl total ROS activity.
  • Storage Instruction:
  • Store the Component A at -20°C and avoid exposure to light. Store the Component B&C at 4°C.
  • Note:
  • Result Data
  • Detection of ROS in Jurkat cells. Jurkat cells were seeded on the same day at 300000 cells/100 uL/well in a Costar black wall/clear bottom 96-well plate. The ROS Red assay stain solution (100 uL/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. The cells were treated with or without 1mM H2O2 for 2 hours. The fluorescence signal was monitored at Ex/Em = 520/605 nm (cut off = 590 nm) with bottom read mode using FlexStation.


  • Suitable Sample:
  • Cell Culture
  • Sample Volume:
  • 96 well: 100 uL, 384 well: 25 uL
  • Label:
  • Fluorometric
  • Detection Method:
  • 520/605 nm
  • Regulation Status:
  • For research use only (RUO)
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