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Trypsin protease activity was analyzed by using Protease Activity Assay Kit. Protease substrate was incubated with 3 units of trypsin in the kit assay buffer. The control wells had protease substrate only (without trypsin). The fluorescence signal was measured starting from time 0 when trypsin was added with a filter set of Ex/Em = 540/590 nm. Samples were done in triplicate.