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Detection of caspase 3/7 Activity in Jurkat cells. Jurkat cells were seeded on the same day at 80,000 cells /well/90 ul in a black wall/clear bottom 96-well costar plate. The cells were treated with or without 20 uM of camptothecin for 5 hours, and/or 5 uM caspase 3/7 inhibitor AC-DEVD-CHO for 10 minutes. The caspase 3/7 assay solution (100 ul/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 490/525 nm.