DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric) is suitable for measuring total DNMT activity or inhibition using nuclear extracts or purified enzymes from a broad range of species such as mammalians, plants, fungi, bacteria, and viruses in a variety of forms including, but not limited to, cultured cells and fresh/frozen tissues.
Quality Control Testing:
Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction:
Store MU3, MU4, MU6 and MU7 at -20°C away from light. Store MU1, MU5, MU8 and 8-Well Assay Strips at 4°C away from light. Store all other components at room temperature away from light. Aliquot to avoid repeated freezing and thawing.
Note:
Result Data
Positive control by using nuclear extract from MCF-7 cells.
The amount of nuclear extracts for each assay can be between 0.5 ug to 20 ug with an optimal range of 5-10 ug. The amount of purified enzymes can be 0.5 ng to 200 ng, depending on the purity and catalytic activity of the enzymes.