DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric) is use for screening DNMT inhibitors.
0.5 to 20 ug nuclear extract, 0.5 to 200 ng purified enzyme
Limit of Detection:
0.5 ug nuclear extract or 0.5 ng purified enzymes
Quality Control Testing:
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Store MU3, MU4, MU6 and MU7 at -20°C away from light. Store MU1, MU5, MU8 and 8-Well Assay Strips at 4°C away from light. Store all other components at room temperature away from light. Aliquot to avoid repeated freezing and thawing.
Positive control by using nuclear extract from MCF-7 cells.