Hydroxymethylated DNA Quantification Kit (Colorimetric)
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The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Demonstration of 5-hydroxymethylcytosine in human brain and colon tissues with 200 ng DNA from different tissues or cells shows OD intensity (A) and percentage of hydroxymethylated DNA (B).
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Specification
Product Description
Hydroxymethylated DNA Quantification Kit (Colorimetric) is used for measuring hydroxymethylated DNA.
Suitable Sample
DNA Extract
Sample Volume
1-8 uL (200 ng include)
Detection Method
Colorimetric
Calibration Range
0.5 to 10 ng/uL
Limit of Detection
40 pg hydroxymethylated DNA
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store HC3, HC4, HC5, HC7 and HC8 at -20°C away from light. Store HC1, HC6, HC9 and 8-Well Assay Strips at 4°C away from light. Store all other components at room temperature away from light.
Note
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Applications
Quantification
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