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Last updated: 2016/12/4
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Multi-Parameter Apoptosis Assay Kit

  • Catalog # : KA1335
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  • Specification
  • Product Description:
  • Multi-Parameter Apoptosis Assay Kit is a measurement for detecting apoptosis cells.
  • Storage Instruction:
  • Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Immunofluorescence
  • Immunofluorescence
  • Staurosporine induces apoptosis in Jurkat cells, as measured by nuclear morphology, a decrease in mitochondrial membrane potential, and an increase of Annexin V FITC positive cells.
    Panels A-C show same field of cells from the control group whereas panels D-F show the same field of cells from the staurosporine-treated group.
    Hoechst staining shows that most control cells have round and intact nuclei (Panel A) whereas staurosporine-treated cells mostly have condensed and fragmented nuclei (Panel D). TMRE staining reveals that most control cells have undisrupted mitochondrial membrane potential (Panel B) whereas staurosporine-treated cells mostly have diminished membrane potential and were not stained (Panel E). Most control cells are Annexin V negative (Panel C) whereas cells treated with staurosporine are mostly Annexin V positive (Panel F), indicating cells are undergoing apoptosis.
  • Functional Study
  • Flow Cytometry
  • Flow Cytometry
  • Staurosporine induces apoptosis in Jurkat cells, as measured by nuclear morphology, a decrease in mitochondrial membrane potential, and an increase of Annexin V FITC positive cells.
    Panels A-C show same field of cells from the control group whereas panels D-F show the same field of cells from the staurosporine-treated group.
    Hoechst staining shows that most control cells have round and intact nuclei (Panel A) whereas staurosporine-treated cells mostly have condensed and fragmented nuclei (Panel D). TMRE staining reveals that most control cells have undisrupted mitochondrial membrane potential (Panel B) whereas staurosporine-treated cells mostly have diminished membrane potential and were not stained (Panel E). Most control cells are Annexin V negative (Panel C) whereas cells treated with staurosporine are mostly Annexin V positive (Panel F), indicating cells are undergoing apoptosis.
  • Application Image
  • Functional Study
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