Effect of staurosporine on mitochondrial potential in Jurkat cells. Jurkat cells were plated at a density of 5 X 104 cells/well. The next day, cells were treated with 2.5 ug/mL of staurosporine or vehicle for two hours in a CO2 incubator at 37°C. JC-1 Staining Solution was then added to the wells and staining was visualized after a 15 minute incubation. Panel A : untreated cells showing most of cells had strong J-aggregation (red). Panel B : staurosporine treated cells showing a majority of cells stained green due to low ΔΨm.