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Last updated: 2017/1/15
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JC-1 Mitochondrial Membrane Potential Assay Kit

  • Catalog # : KA1324
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  • Specification
  • Product Description:
  • JC-1 Mitochondrial Membrane Potential Assay Kit is a fluorescence-based method for tracing the behavior of mitochondria.
  • Storage Instruction:
  • Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.
  • Regulation Status:
  • For research use only (RUO)
  • Publication Reference
  • Applications
  • Immunofluorescence
  • Immunofluorescence
  • Effect of staurosporine on mitochondrial potential in Jurkat cells.
    Jurkat cells were plated at a density of 5 X 104 cells/well. The next day, cells were treated with 2.5 ug/mL of staurosporine or vehicle for two hours in a CO2 incubator at 37°C.
    JC-1 Staining Solution was then added to the wells and staining was visualized after a 15 minute incubation.
    Panel A : untreated cells showing most of cells had strong J-aggregation (red).
    Panel B : staurosporine treated cells showing a majority of cells stained green due to low ΔΨm.
  • Functional Study
  • Flow Cytometry
  • Application Image
  • Functional Study
  • Flow Cytometry
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