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Cholesterol Cell-Based Detection Assay Kit

  • Catalog # : KA1304
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  • Specification
  • Product Description:
  • Cholesterol Cell-Based Detection Assay Kit is a fluorescence-labeled method for histochemical identification of unesterified cholesterol in vivo and in vitro.
  • Storage Instruction:
  • Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.
  • Regulation Status:
  • For research use only (RUO)
  • Publication Reference
  • Applications
  • Immunofluorescence
  • Immunofluorescence
  • Accumulation of cholesterol inside HepG2 cells in response to 1.25 uM U18666A. HepG2 cells were seeded in a 96-well plate at a density of 3 X 104 cells/well and cultured overnight. The next day, cells were treated with DMSO (vehicle) or 1.25 uM U18666A for 48 hours.
    Panel A : Cells treated with DMSO alone demonstrate that majority of cholesterol is localized on the plasma membrane.
    Panel B : U18666A treatment for 48 hours induces intracellular accumulation of cholesterol droplets, indicating blockage of intracellular cholesterol transport.
  • Functional Study
  • Application Image
  • Functional Study
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