CFSE Cell Division Assay Kit
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Flow Cytometry
CD4+ T cells proliferate in response to T cell receptor antibodies and mitogenic stimuli. Murine splenocytes were labeled with CFSE according to the protocol in this booklet. Labeled cells were cultured with no stimulus (red histogram), plate-bound anti-CD3 with soluble anti-CD28 (both 1 ug/mL; purple histogram) or Concanavalin A (5 ug/mL; green histogram). At the indicated time points, cells were stained with fluorochrome-conjugated anti-CD4 and analyzed on a Miltenyi MACSQuant cyometer. FlowJo analysis software was used to gate on CD4+ T cells and generate the overlaid histogram plots.
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Specification
Product Description
CFSE Cell Division Assay Kit is a fluorescence-based method for tracing cell divisions.
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C. For best results, please store the components as described in the protocol.
Avoid repeated freezing and thawing. -
Applications
Flow Cytometry
CD4+ T cells proliferate in response to T cell receptor antibodies and mitogenic stimuli. Murine splenocytes were labeled with CFSE according to the protocol in this booklet. Labeled cells were cultured with no stimulus (red histogram), plate-bound anti-CD3 with soluble anti-CD28 (both 1 ug/mL; purple histogram) or Concanavalin A (5 ug/mL; green histogram). At the indicated time points, cells were stained with fluorochrome-conjugated anti-CD4 and analyzed on a Miltenyi MACSQuant cyometer. FlowJo analysis software was used to gate on CD4+ T cells and generate the overlaid histogram plots.
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