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Last updated: 2017/4/23

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Autophagy/Cytotoxicity Dual Staining Kit

  • Catalog # : KA1299
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  • Specification
  • Product Description:
  • Autophagy/Cytotoxicity Dual Staining Kit is a fluorescence-labeled method for screening autophagy and cytotoxicity at the cellular level.
  • Storage Instruction:
  • Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Immunofluorescence
  • Immunofluorescence
  • Tamoxifen increases autophagy but not cell death in HepG2 cells as measured by fluorescence microscopy.
    Panel A : MDC staining of HepG2 cells treated with vehicle. There is a basal level of autophagy, indicated by faint silver dot staining of autophagic vacuoles.
    Panel B : Propidium iodide staining of HepG2 cells treated with vehicle. There are few dead cells with only background staining of propidium iodide.
    Panel C : MDC staining of HepG2 cells treated with 10 uM Tamoxifen.
    Panel D : Propidium iodide staining of HepG2 cells treated with 10 uM Tamoxifen, showing a similar staining pattern to that of cells treated with vehicle.
  • Functional Study
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  • Functional Study
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