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Last updated: 2021/4/11
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7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit 

  • Catalog # : KA1293
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  • Specification
  • Product Description:
  • 7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit is a fluorescence-labeled method for determining the cytotoxic effect of immune effector cells at a single cell level.
  • Storage Instruction:
  • Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.
  • Regulation Status:
  • For research use only (RUO)
  • Applications
  • Flow Cytometry
  • Flow Cytometry
  • The degree of cytotoxicity of natural killer cells (effector cells; NK cells) on K-562 cells (target cells) depends on the ratio of NK cells to K-562 cells. K-562 cells were labeled with CFSE and co-cultured with NK cells at the ratios indicated above. Cells were co-cultured for four hours at 37°C. At the end of the experiment, dead cells were labeled with 7-AAD. The percentage of K-562 cell death was analyzed by a flow cytometer. When K-562 cells were cultured alone, there is little cell death (2%). Addition of NK cells at a ratio as low as 6.25 : 1 caused a significant increase in K-562 cell death (8.2%). At a ratio of NK cells to target cells of 25 : 1, there is 19.7% death of K-562 cells.
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