KNG1 (Human) ELISA Kit
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Specification
Product Description
KNG1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human KNG1 in urine and cell culture supernatants.
Suitable Sample
Cell Culture Supernatant, Milk, Saliva, Urine
Sample Volume
50 uL
Label
HRP-conjugate
Detection Method
Colorimetric
Assay Type
Quantitative
Intra-Assay
4.80%
Inter-Assay
7.30%
Spiking Recovery
94%
Calibration Range
1.56 to 100 ng/mL
Reactivity
Human
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store components of the kit at 4°C or -20°C as described in the protocol.
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Applications
Quantification
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Gene Info — KNG1
Entrez GeneID
3827Gene Name
KNG1
Gene Alias
BDK, KNG
Gene Description
kininogen 1
Omim ID
228960Gene Ontology
HyperlinkGene Summary
High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein (see MIM 147910)-kinin system. The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing. Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3. However, HMWK contains a 56-kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4-kD light chain that consists of domain 5L. In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide. BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin (see MIM 601699) from endothelial cells. During vascular damage, BK stimulates smooth muscle proliferation and intimal hypertrophy. Release of BK from HMWK generates a 2-chain HMWK, termed HMWKa, containing the heavy and light chains joined by a disulfide bond (Merkulov et al., 2008 [PubMed 18000168]).[supplied by OMIM
Other Designations
alpha-2-thiol proteinase inhibitor|bradykinin
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Interactome
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Publication Reference
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Regulatory effects of ferritin on angiogenesis.
Coffman LG, Parsonage D, D'Agostino R Jr, Torti FM, Torti SV.
PNAS 2009 Jan; 106(2):570.
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Antithrombotic activity of kininogen is mediated by inhibitory effects of domain 3 during arterial injury in vivo.
Hassan S, Sainz IM, Khan MM, Bradford HN, Isordia-Salas I, Kashem SW, Sartor RB, Colman RW.
American Journal of Physiology. Heart and Circulatory Physiology 2007 Jun; 292(6):H2959.
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Regulatory effects of ferritin on angiogenesis.
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