APOB (Human) ELISA Kit
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Specification
Product Description
APOB (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human APOB.
Suitable Sample
Cell Culture Supernatants, Cell Lysate, CSF, Plasma, Serum
Sample Volume
50 uL
Label
Peroxidase-conjugated
Detection Method
Colorimetric
Assay Type
Quantitative
Calibration Range
3.906 to 250 ng/mL
Reactivity
Human
Regulation Status
For research use only (RUO)
Storage Instruction
Store components of the kit at 4°C or -20°C as described in the protocol.
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Applications
Quantification
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Gene Info — APOB
Entrez GeneID
338Gene Name
APOB
Gene Alias
FLDB
Gene Description
apolipoprotein B (including Ag(x) antigen)
Gene Ontology
HyperlinkGene Summary
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq
Other Designations
OTTHUMP00000115994|apoB-100|apoB-48|apolipoprotein B|apolipoprotein B48
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Interactome
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Disease
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Publication Reference
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iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy.
Jun Bai, Jia-Yong Zhong, Wang Liao, Ruo Hu, Liang Chen, Xian-Jin Wu, Shuang-Ping Liu.
Molecular MedicineRreports 2020 Nov; 22(5):3607.
Application:Quant, Human, Human serum.
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Greater expression of postprandial inflammatory genes in humans after intervention with saturated when compared to unsaturated fatty acids.
Monfort-Pires M, Crisma AR, Bordin S, Ferreira SRG.
European Journal of Nutrition 2017 Nov; [Epub].
Application:Quant, Human, Human blood.
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Novel biomarkers of cardiometabolic risk are associated with plasma glucose within non-diabetic range. The Brazilian Longitudinal Study of Adult Health-ELSA-Brazil.
Almeida-Pititto B, Ribeiro-Filho FF, Lotufo PA, Bensenor IM, Ferreira SR.
Diabetes Research and Clinical Practice 2015 Jul; 109(1):110.
Application:ELISA, Human, Plasma.
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Cideb Facilitates the Lipidation of Chylomicrons in the Small Intestine.
Zhang LJ, Wang C, Yuan Y, Wang H, Wu J, Liu F, Li L, Gao X, Zhao YL, Hu PZ, Li P, Ye J.
Journal of Lipid Research 2014 Jul; 55(7):1279.
Application:ELISA, Human, Supernatants collected from Caco-2 cells culture medium.
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The many intersecting pathways underlying apolipoprotein B secretion and degradation.
Brodsky JL, Fisher EA.
rends in Endocrinology and Metabolism: TEM 2008 Sep; 19(7):254.
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Apolipoprotein B as a long-term predictor of mortality in type 1 diabetes mellitus: a 15-year follow up.
Stettler C, Suter Y, Allemann S, Zwahlen M, Christ ER, Diem P.
Journal of Internal Medicine 2006 Sep; 260(3):272.
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iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy.
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