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Last updated: 2021/1/10
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Caspase-3 Assay Kit (Colorimetric) 

  • Catalog # : KA0740
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  • Specification
  • Product Description:
  • Caspase-3 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
  • Reactivity:
  • Mammals
  • Storage Instruction:
  • Store the kit at -20°C.
  • Note:
  • Result Data
  • Induction of Caspase-3 Activity by Anti-Fas antibody in Jurkat-T cells using Caspase-3 Assay Kit (Colorimetric) (Cat # KA0740).


  • Suitable Sample:
  • Cell Lysate
  • Detection Method:
  • Colorimetric
  • Regulation Status:
  • For research use only (RUO)
  • Publication Reference
  • Applications
  • Functional Study
  • Application Image
  • Functional Study
  • Gene Information
  • Entrez GeneID:
  • 836
  • Gene Name:
  • CASP3
  • Gene Alias:
  • CPP32,CPP32B,SCA-1
  • Gene Description:
  • caspase 3, apoptosis-related cysteine peptidase
  • Gene Summary:
  • This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000165054,PARP cleavage protease,SREBP cleavage activity 1,Yama,apopain,caspase 3,caspase 3, apoptosis-related cysteine protease,cysteine protease CPP32,procaspase3
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