ACE (Human) ELISA Kit
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Specification
Product Description
ACE (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human ACE.
Suitable Sample
Cell Culture Supernatant, Plasma (heparin), Serum, Saliva
Sample Volume
100 uL
Label
HRP-conjugated
Detection Method
Colorimetric
Assay Type
Quantitative
Calibration Range
0.78 to 50 ng/mL
Limit of Detection
< 5 pg/mL
Reactivity
Human
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
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Applications
Quantification
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Gene Info — ACE
Entrez GeneID
1636Gene Name
ACE
Gene Alias
ACE1, CD143, DCP, DCP1, MGC26566
Gene Description
angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined. [provided by RefSeq
Other Designations
CD143 antigen|angiotensin I converting enzyme|angiotensin converting enzyme, somatic isoform|carboxycathepsin|dipeptidyl carboxypeptidase 1|kininase II|peptidase P|peptidyl-dipeptidase A|testicular ECA
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Interactome
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Pathway
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Disease
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Publication Reference
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Selective restoration of male fertility in mice lacking angiotensin-converting enzymes by sperm-specific expression of the testicular isozyme.
Ramaraj P, Kessler SP, Colmenares C, Sen GC.
The Journal of Clinical Investigation 1998 Jul; 102(2):371.
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Molecular cloning of human testicular angiotensin-converting enzyme: the testis isozyme is identical to the C-terminal half of endothelial angiotensin-converting enzyme.
Ehlers MR, Fox EA, Strydom DJ, Riordan JF.
PNAS 1989 Oct; 86(20):7741.
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Selective restoration of male fertility in mice lacking angiotensin-converting enzymes by sperm-specific expression of the testicular isozyme.
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