12(S)-HETE ELISA Kit
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Specification
Product Description
12(S)-HETE ELISA Kit is a competitive immunoassay for the quantitative determination of 12(S)-HETE in biological fluids.
Suitable Sample
Cell Culture Supernatant, Plasma
Sample Volume
100 uL
Label
AP-conjugate
Detection Method
Colorimetric
Assay Type
Quantitative
Intra-Assay
10.26%
Inter-Assay
11.30%
Spiking Recovery
Cell Culture Supernatant: 94.00%, Plasma (Heparin): 104.00%, Plasma (EDTA): 97.00%
Calibration Range
195 to 50000 pg/mL
Limit of Detection
146 pg/mL
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
The standard and conjugate must be stored at -20°C; others store at 4°C.
Avoid repeated freezing and thawing. -
Applications
Quantification
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Publication Reference
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2,3',4,5'-Tetramethoxystilbene prevents deoxycorticosterone-salt-induced hypertension: contribution of cytochrome P-450 1B1.
Sahan-Firat S, Jennings BL, Yaghini FA, Song CY, Estes AM, Fang XR, Farjana N, Khan AI, Malik KU.
American Journal of Physiology. Heart and Circulatory Physiology 2010 Dec; 299(6):H1891.
Application:Quant, Rat, Rat plasma.
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2,3',4,5'-Tetramethoxystilbene prevents deoxycorticosterone-salt-induced hypertension: contribution of cytochrome P-450 1B1.
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