PDK1 Kinase (Human) Assay/Inhibitor Screening Assay Kit is a single-site, non-quantitative immunoassay for PDK1 activity. Plates are pre-coated with a substrate-peptide corresponding to surrounding AKT1 T308, which contains threonine residues that can be efficiently phosphorylated by PDK1. The detector antibody specifically detects only the phosphorylated form of threonine residue on substrate-peptide.
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant PDK1 enzyme reaction.