The AKT/PKB kinase Assay/Inhibitor Screening kit is a single-site, non-quantitative immunoassay for AKT activity. Plates are pre-coated with "AKTide-2T" (10), which can be efficiently phosphorylated by AKT1, 2 and 3 on a microtiter plate. The detector antibody is AT-3E2, an antibody that specifically detects only the phosphorylated "AKTide-2T".
Storage Instruction:
Store the kit at 2 - 8°C.
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant AKT enzyme reaction.