MAPKAP-kinase 2 (Human) Assay/Inhibitor Screening Assay Kit is a single-site, non-quantitative immunoassay for MAPKAP-kinase 2 activity. Plates are pre-coated with a substrate corresponding to recombinant LSP1 (Leukocyte Specific Protein), which contains a serine residue that are phosphorylated by MAPKAP-kinase 2 (MAPK-activated protein kinase 2). The detector antibody specifically detects only the phosphorylated form of serine-204 on LSP1.
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant MAPKAP-kinase 2 enzyme reaction.
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq