Polo-like kinase 1 (Human) Assay/Inhibitor Screening Assay Kit is a single-site, non-quantitative immunoassay for Plk1 activity. Plates are pre-coated with a substrate corresponding to recombinant Protein-X, which contains threonine residues that can be efficiently phosphorylated by Plk1. The detector antibody specifically detects only the phosphorylated form of threonine residue on Protein-X.
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant Plk1 enzyme reaction.