Checkpoint Kinase (Human) Assay/Inhibitor Screening Assay Kit is a single-site, non-quantitative immunoassay for checkpoint kinase activity. Plates are pre-coated with a substrate corresponding to recombinant Cdc25C, which contains serine residues that can be phosphorylated by checkpoint kinases, including Chk1, Chk2 and C-TAK1. The detector antibody specifically detects only the phosphorylated form of serine 216 residue on Cdc25C.
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant Chk1 enzyme reaction.