TrkA Kinase (Human) Assay/Inhibitor Screening Kit is a single-site, non-quantitative immunoassay for kinase activity of recombinant catalytic domain of TrkA. Plates are pre-coated with a newly designed "Tyrosine kinase-substrate-1", which can be easily phosphorylated by recombinant catalytic domain of TrkA. The detector antibody is PY-39, an antibody that specifically detects the phosphotyrosine residue on "Tyrosine kinase-substrate-1".
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant TrkA catalytic domain enzyme reaction.
This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq
OTTHUMP00000038736,Oncogene TRK,high affinity nerve growth factor receptor,tyrosine kinase receptor A