The p56Lck Kinase Assay/Inhibitor Screening kit is a single-site, non-quantitative immunoassay for kinase activity of recombinant catalytic domain of Lck. Plates are pre-coated with a newly designed "Tyrosine kinase-binding module-1", which can easily bind recombinant catalytic domain of Lck, subsequently activate Lck kinase activity on a microtiter plate. The detector antibody is PY-39, an antibody that specifically detects the phosphotyrosine residue on recombinant catalytic domain of Lck itself, which means that this kit measures the intensity of autophosphorylation of Lck catalytic domain.
Storage Instruction:
Store the kit at 2 - 8°C.
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant Lck catalytic domain enzyme reaction.
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