p56Lck Kinase (Human) Assay/Inhibitor Screening Assay Kit is a single-site, non-quantitative immunoassay for kinase activity of recombinant catalytic domain of Lck. Plates are pre-coated with a newly designed "Tyrosine kinase-binding module-1", which can easily bind recombinant catalytic domain of Lck, subsequently activate Lck kinase activity on a microtiter plate. The detector antibody is PY-39, an antibody that specifically detects the phosphotyrosine residue on recombinant catalytic domain of Lck itself, which means that this kit measures the intensity of autophosphorylation of Lck catalytic domain.
Quality Control Testing:
Dose dependent curve
Dose dependent curve of recombinant Lck catalytic domain enzyme reaction.
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq
T-lymphocyte specific protein tyrosine kinase p56lck,p56(LSTRA) protein-tyrosine kinase,protein tyrosine kinase,proto-oncogene tyrosine-protein kinase LCK